A Method for Combining Isolates of Phytophthora sojae to Screen for Novel Sources of Resistance to Phytophthora Stem and Root Rot in Soybean.
Identifieur interne : 000D30 ( Main/Exploration ); précédent : 000D29; suivant : 000D31A Method for Combining Isolates of Phytophthora sojae to Screen for Novel Sources of Resistance to Phytophthora Stem and Root Rot in Soybean.
Auteurs : R L Matthiesen [États-Unis] ; N S Abeysekara ; J J Ruiz-Rojas ; R M Biyashev ; M A Saghai Maroof ; A E Robertson [États-Unis]Source :
- Plant disease [ 0191-2917 ] ; 2016.
Abstract
Soybean cultivars with specific single resistance genes (Rps) are grown to reduce yield loss due to Phytophthora stem and root rot caused by the oomycete pathogen Phytophthora sojae. To identify novel Rps loci, soybean lines are often screened several times, each time with an isolate of P. sojae that differs in virulence on various Rps genes. The goal of this study was to determine whether several isolates of P. sojae that differ in virulence on Rps genes could be combined into a single source of inoculum and used to screen soybean lines for novel Rps genes. A set of 14 soybean differential lines, each carrying a specific Rps gene, was inoculated with three isolates of P. sojae, which differed in virulence on 6 to 10 Rps genes, individually or in a 1:1:1 mixture. Inoculum containing the 1:1:1 mixture of isolates was virulent on 13 Rps genes. The mixed-inoculum method was used to screen 1,019 soybean accessions in a blind assay for novel sources of resistance. In all, 17% of Glycine max accessions and 11% of G. soja accessions were resistant (≤30% dead plants), suggesting that these accessions may carry a novel Rps gene or genes. Advantages of combining isolates into a single source of inoculum include reduced cost, ability to screen soybean germplasm with inoculum virulent on all known Rps genes, and ease of identifying novel sources of resistance. This study is a precursor to identifying novel sources of resistance to P. sojae in soybean using RXLR effectors.
DOI: 10.1094/PDIS-08-15-0916-RE
PubMed: 30686197
Affiliations:
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sort="Abeysekara, N S" uniqKey="Abeysekara N" first="N S" last="Abeysekara">N S Abeysekara</name><affiliation><nlm:affiliation>Department of Plant and Soil Sciences, University of Delaware, Newark 19716.</nlm:affiliation><wicri:noCountry code="subField">Newark 19716</wicri:noCountry></affiliation></author><author><name sortKey="Ruiz Rojas, J J" sort="Ruiz Rojas, J J" uniqKey="Ruiz Rojas J" first="J J" last="Ruiz-Rojas">J J Ruiz-Rojas</name><affiliation><nlm:affiliation>Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg 24060.</nlm:affiliation><wicri:noCountry code="subField">Blacksburg 24060</wicri:noCountry></affiliation></author><author><name sortKey="Biyashev, R M" sort="Biyashev, R M" uniqKey="Biyashev R" first="R M" last="Biyashev">R M Biyashev</name><affiliation><nlm:affiliation>Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg 24060.</nlm:affiliation><wicri:noCountry code="subField">Blacksburg 24060</wicri:noCountry></affiliation></author><author><name sortKey="Maroof, M A Saghai" sort="Maroof, M A Saghai" uniqKey="Maroof M" first="M A Saghai" last="Maroof">M A Saghai Maroof</name><affiliation><nlm:affiliation>Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg 24060.</nlm:affiliation><wicri:noCountry code="subField">Blacksburg 24060</wicri:noCountry></affiliation></author><author><name sortKey="Robertson, A E" sort="Robertson, A E" uniqKey="Robertson A" first="A E" last="Robertson">A E Robertson</name><affiliation wicri:level="4"><nlm:affiliation>Department of Plant Pathology and Microbiology, Iowa State University.</nlm:affiliation><country>États-Unis</country><placeName><settlement type="city">Ames (Iowa)</settlement><region type="state">Iowa</region></placeName><orgName type="university">Université d'État de l'Iowa</orgName></affiliation></author></analytic><series><title level="j">Plant disease</title><idno type="ISSN">0191-2917</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Soybean cultivars with specific single resistance genes (Rps) are grown to reduce yield loss due to Phytophthora stem and root rot caused by the oomycete pathogen Phytophthora sojae. To identify novel Rps loci, soybean lines are often screened several times, each time with an isolate of P. sojae that differs in virulence on various Rps genes. The goal of this study was to determine whether several isolates of P. sojae that differ in virulence on Rps genes could be combined into a single source of inoculum and used to screen soybean lines for novel Rps genes. A set of 14 soybean differential lines, each carrying a specific Rps gene, was inoculated with three isolates of P. sojae, which differed in virulence on 6 to 10 Rps genes, individually or in a 1:1:1 mixture. Inoculum containing the 1:1:1 mixture of isolates was virulent on 13 Rps genes. The mixed-inoculum method was used to screen 1,019 soybean accessions in a blind assay for novel sources of resistance. In all, 17% of Glycine max accessions and 11% of G. soja accessions were resistant (≤30% dead plants), suggesting that these accessions may carry a novel Rps gene or genes. Advantages of combining isolates into a single source of inoculum include reduced cost, ability to screen soybean germplasm with inoculum virulent on all known Rps genes, and ease of identifying novel sources of resistance. This study is a precursor to identifying novel sources of resistance to P. sojae in soybean using RXLR effectors.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">30686197</PMID><DateRevised><Year>2019</Year><Month>11</Month><Day>20</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0191-2917</ISSN><JournalIssue CitedMedium="Print"><Volume>100</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Plant disease</Title><ISOAbbreviation>Plant Dis</ISOAbbreviation></Journal><ArticleTitle>A Method for Combining Isolates of Phytophthora sojae to Screen for Novel Sources of Resistance to Phytophthora Stem and Root Rot in Soybean.</ArticleTitle><Pagination><MedlinePgn>1424-1428</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1094/PDIS-08-15-0916-RE</ELocationID><Abstract><AbstractText>Soybean cultivars with specific single resistance genes (Rps) are grown to reduce yield loss due to Phytophthora stem and root rot caused by the oomycete pathogen Phytophthora sojae. To identify novel Rps loci, soybean lines are often screened several times, each time with an isolate of P. sojae that differs in virulence on various Rps genes. The goal of this study was to determine whether several isolates of P. sojae that differ in virulence on Rps genes could be combined into a single source of inoculum and used to screen soybean lines for novel Rps genes. A set of 14 soybean differential lines, each carrying a specific Rps gene, was inoculated with three isolates of P. sojae, which differed in virulence on 6 to 10 Rps genes, individually or in a 1:1:1 mixture. Inoculum containing the 1:1:1 mixture of isolates was virulent on 13 Rps genes. The mixed-inoculum method was used to screen 1,019 soybean accessions in a blind assay for novel sources of resistance. In all, 17% of Glycine max accessions and 11% of G. soja accessions were resistant (≤30% dead plants), suggesting that these accessions may carry a novel Rps gene or genes. Advantages of combining isolates into a single source of inoculum include reduced cost, ability to screen soybean germplasm with inoculum virulent on all known Rps genes, and ease of identifying novel sources of resistance. This study is a precursor to identifying novel sources of resistance to P. sojae in soybean using RXLR effectors.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Matthiesen</LastName><ForeName>R L</ForeName><Initials>RL</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology and Microbiology, Iowa State University, Ames 50011.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Abeysekara</LastName><ForeName>N S</ForeName><Initials>NS</Initials><AffiliationInfo><Affiliation>Department of Plant and Soil Sciences, University of Delaware, Newark 19716.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ruiz-Rojas</LastName><ForeName>J J</ForeName><Initials>JJ</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg 24060.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Biyashev</LastName><ForeName>R M</ForeName><Initials>RM</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg 24060.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Maroof</LastName><ForeName>M A Saghai</ForeName><Initials>MAS</Initials><AffiliationInfo><Affiliation>Department of Crop and Soil Environmental Sciences, Virginia Tech, Blacksburg 24060.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Robertson</LastName><ForeName>A E</ForeName><Initials>AE</Initials><Identifier Source="ORCID">http://orcid.org/0000-0003-4281-6350</Identifier><AffiliationInfo><Affiliation>Department of Plant Pathology and Microbiology, Iowa State University.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2016</Year><Month>04</Month><Day>04</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Plant Dis</MedlineTA><NlmUniqueID>9882809</NlmUniqueID><ISSNLinking>0191-2917</ISSNLinking></MedlineJournalInfo></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2019</Year><Month>1</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2016</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>7</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">30686197</ArticleId><ArticleId IdType="doi">10.1094/PDIS-08-15-0916-RE</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>Iowa</li></region><settlement><li>Ames (Iowa)</li></settlement><orgName><li>Université d'État de l'Iowa</li></orgName></list><tree><noCountry><name sortKey="Abeysekara, N S" sort="Abeysekara, N S" uniqKey="Abeysekara N" first="N S" last="Abeysekara">N S Abeysekara</name><name sortKey="Biyashev, R M" sort="Biyashev, R M" uniqKey="Biyashev R" first="R M" last="Biyashev">R M Biyashev</name><name sortKey="Maroof, M A Saghai" sort="Maroof, M A Saghai" uniqKey="Maroof M" first="M A Saghai" last="Maroof">M A Saghai Maroof</name><name sortKey="Ruiz Rojas, J J" sort="Ruiz Rojas, J J" uniqKey="Ruiz Rojas J" first="J J" last="Ruiz-Rojas">J J Ruiz-Rojas</name></noCountry><country name="États-Unis"><region name="Iowa"><name sortKey="Matthiesen, R L" sort="Matthiesen, R L" uniqKey="Matthiesen R" first="R L" last="Matthiesen">R L Matthiesen</name></region><name sortKey="Robertson, A E" sort="Robertson, A E" uniqKey="Robertson A" first="A E" last="Robertson">A E Robertson</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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